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Reagents and additives

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In addition to culture media, additives and reagents have been developed to improve the cultivation of microorganisms or cell cultures of higher organisms. The products are sterile, are offered with a certificate of analysis for each batch guaranteeing high product quality. They are delivered both to scientific laboratories and to production sites, and are manufactured in accordance with ISO9001.

Growth Additives

Name Volume LT Biotech catalog number
Sodium Pyruvate (100 mM) 100 ml LTGA01
L-Glutamine (200 mM) 100 ml LTGA02
MEM Nonessential Amino Acids (100x) 100 ml LTGA03
Stable Glutamine (200 mM) 100 ml LTGA04
NCS21 Supplement (50x), Serum-free 10 ml LTGA05
Hybridoma Supplement, Serum-free 50 ml LTGA06
ITS (100x), Insulin, Transferrin, Selenium 10 ml LTGA07
N2 Supplement (100x), Serum-free 5 ml LTGA08
Insulin human recombinant Solution (5 mg/ml) 5 ml HRINS5ML
Glucose Solution (250 g/L) 50 ml GLUS50ML

Antibiotics

Name Volume LT Biotech catalog number
Amphotericin B Solution (100x) 100 ml LTAnB-L01
Gentamicin Solution, 10 mg/ml 100 ml LTAnB-L02
MycoXpert, Mycoplasma Removal Reagent (50x) 100 ml LTAnB-L03
Penicillin/Streptomycin (100x) 100 ml LTAnB-L04
Penicillin/Streptomycin (100x), with L-Glutamine 100 ml LTAnB-L05
Antibiotic/Antimycotic Solution (100x) 100 ml LTAnB-L06

Separation Media

Name Volume LT Biotech catalog number
Lymphocyte Separation Medium, Density 1.077 g/ml 500 ml LTSM500
Lymphocyte Separation Medium, Density 1.077 g/ml 100 ml LTSM100

Products for cytogenetics

Name Volume LT Biotech catalog number
AminoFirst 100 ml AF100ML
LymphoFirst 100 ml LF100ML
AminoChoise 100 ml AC100ML
MarrowFirst 100 ml MF100ML
Colcemid Solution (10 µg/ml) in DPBS 10 ml LTSMC10
Phytohemagglutinin (PHA-M) 5 ml LTSMC20

Detachment agents

Name Volume LT Biotech catalog number
Accumass 50 ml AccuTop
Accutase Cell Detachment Solution 100 ml LTDe01
Trypsin-EDTA (0.05 %) in DPBS (1x) 100 ml LTDe02
Trypsin-EDTA (0.5 %) in DPBS (10x) 100 ml LTDe03
Trypsin (0.25 %) in DPBS (1x) 100 ml LTDe04
Trypsin (2.5 %) in DPBS (10x) 100 ml LTDe05
Trypsin-EDTA (0.25 %) in HBSS (1x), with phenol red 100 ml TRED1-100ML
Trypsin-EDTA (0.05 %) in HBSS (1x), with phenol red 100 ml TRED2-100ML

Transfection reagents

Name Volume LT Biotech catalog number
Blasticidin S, Powder 100 mg LTTran01
Blasticidin S, Powder 50 mg LTTran02
G-418 Sulfate, Solution, 100 % Activity (50 mg/ml) 100 ml LTTran03
G-418 Sulfate, Solution, 100 % Activity (50 mg/ml) 10 ml LTTran04
G-418 Sulfate, Powder 5 g LTTran05
G-418 Sulfate, Powder 10 g LTTran06
Hygromycin B, Solution (50 mg/ml) 10 ml LTTran07
Puromycin, Powder 100 mg LTTran08
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