The DMEM/F-12 medium is a commonly used basal medium that provides support for the growth of numerous types of cells. The rich blend of this formulation is a result of its composition, which includes DMEM and Ham’s F-12.
The DMEM/F-12 medium is a commonly used basal medium that provides support for the growth of numerous types of cells. The rich blend of this formulation is a result of its composition, which includes DMEM and Ham’s F-12.
No.
Volume:
500 mlAdd to order
Product Specifications
Clear red orange solution
5.5%
Store at +2°C to +8°C protected from light.
Once opened, store at 4° C and use within 6-8 weeks
Ambient
Components | Concentration mg/L |
Amino Acids: | |
Glycine | 18.75 |
L-Alanine | 4.45 |
L-Arginine HCl | 147.50 |
L-Asparagine H2O | 7.50 |
L-Aspartic Acid | 6.65 |
L-Cysteine HCl H2O | 17.56 |
L-Cystine 2 HCl | 31.29 |
L-Glutamic Acid | 7.35 |
L-Glutamine | 365.00 |
L-Histidine HCl H2O | 31.48 |
L-Isoleucine | 54.47 |
L-Leucine | 59.05 |
L-Lysine HCl | 91.25 |
L-Methionine | 17.24 |
L-Phenylalanine | 35.48 |
L-Proline | 17.25 |
L-Serine | 26.25 |
L-Threonine | 53.45 |
L-Tryptophan | 9.02 |
L-Tyrosine Disodium Salt | 48.10 |
L-Valine | 52.85 |
Vitamins: | |
D-Biotin | 0.0035 |
Choline Chloride | 8.98 |
D-Calcium Pantothenate | 2.24 |
Folic Acid | 2.66 |
myo-Inositol | 12.60 |
Nicotinamide | 2.02 |
Pyridoxine HCl | 0.031 |
Pyridoxal HCL | 2.00 |
Riboflavin | 0.219 |
Thiamine HCl | 2.17 |
Vitamin B12 | 0.68 |
Inorganic Salts: | |
CaCl2 2 H2O | 154.50 |
CuSO4 5 H2O | 0.0013 |
Fe(NO3)3 9 H2O | 0.05 |
FeSO4 7 H2O | 0.417 |
KCl | 311.80 |
MgCl2 6 H2O | 61.20 |
MgSO4 | 48.84 |
NaCl | 6996.00 |
NaHCO3 | 1200.00 |
Na2HPO4 | 71.02 |
NaH2PO4 | 54.30 |
ZnSO4 7 H2O | 0.432 |
Other Components: | |
D-Glucose | 3151.00 |
Hypoxanthine | 2.40 |
HEPES | 3574.50 |
Linoleic Acid | 0.042 |
Lipoic Acid | 0.105 |
Phenol Red Sodium Salt | 8.63 |
Putrescine 2 HCl | 0.081 |
Sodium Pyruvate | 55.00 |
Thymidine | 0.365 |
Download:
The DMEM/F-12 medium is a commonly used basal medium that provides support for the growth of numerous types of cells. The rich blend of this formulation is a result of its composition, which includes DMEM and Ham’s F-12.