Hank’s Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as rinsing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. The essential function of a balanced salt solution is to maintain pH and osmotic balance as well as providing cells with water and essential inorganic ions. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation