Hygromycin B is a unique aminoglycoside antibiotic produced by Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin B resistance gene. Resistance to hygromycin B is conferred by the E. coli hygromycin B resistance gene (hyg or hph). Hygromycin B has a different mode of action than G-418 or Blasticidin S selection reagents, making it an excellent choice for dual-selection experiments.

Hygromycin B is used as a selection antibiotic in the concentration range of 200 – 500 µg/ml.


  • Selection antibiotic
  • Hygromycin B phosphotransferase inactivates hygromycin B
  • Transfections with the hyg or hph gene are resistant to hygromycin B
  • Transient and/or stable transfected cells


  • Antibiotic with a broad spectrum of activity
  • High purity
  • High activity
  • Sterile solution