Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

This chemically defined solution is suitable for cell culture. The human insulin was expressed in Saccharomyces cerevisiae.

Applications

  • Additional component for cell culture media
  • Applicable for batch, fed-batch & perfusion culture addition