The medium is versatile enough to support the growth of a diverse range of cells, including non-transformed cells, and is commonly utilized in the production of vaccines, primary pancreatic explants, and lens tissues. Two variants of Media 199 exist, namely M199-Earle and M199-Hank, which are formulated with Earle’s salts and Hank’s salts, respectively. The latter is particularly suitable for atmospheric equilibration.