The medium is versatile enough to support the growth of a diverse range of cells, including non-transformed cells, and is commonly utilized in the production of vaccines, primary pancreatic explants, and lens tissues. Two variants of Media 199 exist, namely M199-Earle and M199-Hank, which are formulated with Earle’s salts and Hank’s salts, respectively. The latter is particularly suitable for atmospheric equilibration.
Products
Medium 199 with Hank’s Salts, with L-Glutamine
No.
LTLM-B30
Volume:
500 mlAdd to order
Product Specifications
Appearance
Clear red orange solution
CO2 concentration, optimum
5.0%
Storage and shelf life
Store at +2°C – +8°C protected from light.
Once opened, store at 4°C and use within 6-8 weeks.
Shipping conditions
Ambient
Formulation
| Components | Concentration mg/L |
| Amino Acids: | |
| L-Alanine | 25.00 |
| L-Arginine HCl | 70.00 |
| L-Aspartic Acid | 30.00 |
| L-Cysteine HCl H2O | 0.11 |
| L-Cystine 2HCl | 26.00 |
| L-Glutamic Acid | 66.80 |
| L-Glutamine | 100.00 |
| Glycine | 50.00 |
| L-Histidine HCl H2O | 21.88 |
| L-Hydroxy-L-Proline | 10.00 |
| L-Isoleucine | 20.00 |
| L-Leucine | 60.00 |
| L-Lysine HCl | 70.00 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 25.00 |
| L-Proline | 40.00 |
| L-Serine | 25.00 |
| L-Threonine | 30.00 |
| L-Tryptophan | 10.00 |
| L-Tyrosine 2Na 2H2O | 57.66 |
| L-Valine | 25.00 |
| Vitamins: | |
| Ascorbic Acid | 0.056 |
| D-Biotin | 0.01 |
| Calciferol | 0.10 |
| Choline chloride | 0.50 |
| D-Calcium Pantothenate | 0.01 |
| Folic Acid | 0.01 |
| Menadione Sodium Bisulfite | 0.016 |
| myo-Inositol | 0.05 |
| Nicotinamide | 0.025 |
| Nicotinic Acid | 0.025 |
| Paraaminobenzoic Acid | 0.05 |
| Pyridoxal HCl | 0.025 |
| Pyridoxine HCl | 0.025 |
| Riboflavin | 0.01 |
| Thiamine HCl | 0.01 |
| Vitamin A (Acetate) | 0.14 |
| (±)-α-Tocopherol Phosphate 2Na | 0.01 |
| Inorganic Salts: | |
| CaCl2 2H2O | 265.00 |
| Fe(NO3)3 9 H2O | 0.72 |
| MgSo4 (anhydrous) | 97.67 |
| KCl | 400.00 |
| Na Acetate (anhydrous) | 50.00 |
| NaHCO3 | 2200.00 |
| NaCl | 6800.00 |
| NaH2PO4 (anhydrous) | 122.00 |
| Other Components: | |
| 2-Deoxy-D-Ribose | 0.50 |
| Adenine Sulfate | 10.00 |
| Adenosine 5‘-Phosphate | 0.24 |
| Adenosine 5‘-Triphosphate 2Na | 1.00 |
| Cholesterol | 0.20 |
| D-Glucose | 1000.00 |
| L-Glutathione (reduced) | 0.05 |
| Guanine HCl | 0.30 |
| Hypoxanthine | 0.30 |
Precautions and Disclaimer
This product is for research use only.Download:
General information in pdf
The medium is versatile enough to support the growth of a diverse range of cells, including non-transformed cells, and is commonly utilized in the production of vaccines, primary pancreatic explants, and lens tissues. Two variants of Media 199 exist, namely M199-Earle and M199-Hank, which are formulated with Earle’s salts and Hank’s salts, respectively. The latter is particularly suitable for atmospheric equilibration.
