N2 Supplement is a serum-free chemically defined supplement based on Bottenstein’s N2 formulation. This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N2 Supplement is provided as a 100x concentrate.

Applications

N2 Supplement can be used in combination with NCS21 or NCS27 Supplement for:

  • Differentiation of ES cells into neuron lineage (neuron and astrocytes).
  • Differentiation of neural stem cells into astrocytes and neurons.
  • Optimal for serum free growth for neuroblastomas.