PlasmidCap Persefose HP is a thiophilic affinity resin made by fixing sulfur-containing compound 2-mercapyridine on high resolution crosslinked agarose. Its optimized ligand density has appropriate affinity with superhelix DNA, and fine particle microspheres can improve the load of superhelix DNA with larger molecular weight. The principle of thiophilic affinity is to use the interaction between electron donor and electron acceptor to separate and purify biomolecules. This force is strengthened in a high salt environment and weakened in a low salt environment.