With its unique composition containing high levels of vitamins and glutathione, RPMI 1640 medium is well-suited for promoting the growth of a wide range of cell types when compared to other media.
Products
RPMI 1640, with L-Glutamine, powder medium, with 25 mM HEPES, w/o Sodium Bicarbonate
No.
LTPM-L12 / 50 L
LTPM-L11 / 10 L
Add to order
Product Specifications
Appearance
Off-white to creamy white, homogenous powder
Solubility
Clear solution at 16.4 g/l
pH w/o Sodium Bicarbonate
6.40 – 7.00
pH with Sodium Bicarbonate
6.70 – 7.30
Osmolality w/o Sodium Bicarbonate
280 – 320 mOsm/kg
Osmolality with Sodium Bicarbonate
320 – 360 mOsm/kg
Endotoxin
< 5 EU/ml
Cell Culture
Tested
Storage
+2°C to +8°C, dry and protected from light
Use at
16.4 g/l
Add
2.0 g/l Sodium Bicarbonate
CO2 concentration optimum for liquid medium
4.5 %
Instructions for Use: Preparation of 1 liter liquid medium
1. Suspend 10.0 g in 900 ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water. 2. Add 3.7 g of sodium bicarbonate powder or 49.3 ml of 7.5 % sodium bicarbonate solution for 1 liter of medium and stir until dissolved. 3. Adjust the pH to 0.2 to 0.3 pH units below the desired pH using 1 N HCl or 1 N NaOH since the pH tends to rise during filtration. 4. Make up the final volume to 1000ml with tissue culture grade water. 5. Sterilize the medium immediately by filtering through a sterile membrane filter with porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide. 6. Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers. 7. Store liquid medium at +2°C to +8°C and in dark until use.Formulation
| Components | mg/l | Vitamins | |
| Amino Acids | p-Amino Benzoic Acid | 1.00 | |
| L-Arginine HCl | 241.00 | D-Biotin | 0.20 |
| L-Asparagine | 50.00 | Choline Chloride | 3.00 |
| L-Aspartic Acid | 20.00 | D-Calcium Pantothenate | 0.25 |
| L-Cystine x 2 HCl | 65.20 | Folic Acid | 1.00 |
| L-Glutamine | 300.00 | myo-Inositol | 35.00 |
| Glycine | 10.00 | Nicotinamide | 1.00 |
| L-Glutamic Acid | 20.00 | Pyridoxine x HCl | 1.00 |
| L-Histidine HCl | 20.96 | Riboflavin | 0.20 |
| L-Hydroxy-L-Proline | 20.00 | Thiamine x HCl | 1.00 |
| L-Isoleucine | 50.00 | Vitamin B12 | 0.005 |
| L-Leucine | 50.00 | Inorganic Salts | |
| L-Lysine x HCl | 40.00 | Calcium Nitrate x 4 H2O | 100.00 |
| L-Methionine | 15.00 | Magnesium Sulfate | 48.84 |
| L-Phenylalanine | 15.00 | Potassium Chloride | 400.00 |
| L-Proline | 20.00 | Sodium Chloride | 6000.00 |
| L-Serine | 30.00 | Sodium Phosphate Dibasic Anhydrous | 800.00 |
| L-Threonine | 20.00 | Other Components | |
| L-Tryptophan | 5.00 | D-Glucose | 2000.00 |
| L-Tyrosine x 2 Na 2 H2O | 28.83 | L-Glutathione Reduced | 1.00 |
| L-Valine | 20.00 | HEPES | 5958.00 |
| Phenol Red Sodium Salt | 5.30 |
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Download:
General information in pdf
With its unique composition containing high levels of vitamins and glutathione, RPMI 1640 medium is well-suited for promoting the growth of a wide range of cell types when compared to other media.
