The SILAC RPMI medium has been designed and optimized for labeling experiments that involve the use of stable amino acid isotopes in cell culture for the purpose of analyzing protein expression through mass spectrometry (MS).
Applications:
• Quantitative and functional proteomics
• Analyses of tissue regeneration
• Analyses of post-translational modifications
• MS (Mass Spectrometry)
• NMR (Nuclear Magnetic Resonance)
No.
Volume:
500 mlAdd to order
Product Specifications
Clear solution
4.5 %
Store at +2°C to +8°C protected from light.
Once opened, store at 4° C and use within 6-8 weeks.
Ambient
| Components | Concentration mg/L |
| Amino Acids: | |
| Glycine | 10.00 |
| L-Asparagine H2O | 56.82 |
| L-Aspartic Acid | 20.00 |
| L-Cystine 2HCl | 65.20 |
| L-Glutamic Acid | 20.00 |
| L-Histidine HCl H2O | 20.27 |
| L-Hydroxy-L-Proline | 20.00 |
| L-Isoleucine | 50.00 |
| L-Leucine | 50.00 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 15.00 |
| L-Proline | 20.00 |
| L-Serine | 30.00 |
| L-Threonine | 20.00 |
| L-Tryptophan | 5.00 |
| L-Tyrosine 2Na 2H2O | 28.83 |
| L-Valine | 20.00 |
| Vitamins: | |
| p-Amino Benzoic Acid | 1.00 |
| D-Biotin | 0.20 |
| Choline Chloride | 3.00 |
| D-Calcium Pantothenate | 0.25 |
| Folic Acid | 1.00 |
| myo-Inositol | 35.00 |
| Nicotinamide | 1.00 |
| Pyridoxine HCl | 1.00 |
| Riboflavin | 0.20 |
| Thiamine HCl | 1.00 |
| Vitamin B12 | 0.005 |
| Inorganic Salts: | |
| Ca(NO3)2 4 H2O | 100.00 |
| KCl | 400.00 |
| MgSO4 7H2O | 100.00 |
| NaCl | 6000.00 |
| NaHCO3 | 2000.00 |
| Na2HPO4 | 800.00 |
| Other Components: | |
| D-Glucose | 2000.00 |
| L-Glutathione Reduced | 1.00 |
Download:
The SILAC RPMI medium has been designed and optimized for labeling experiments that involve the use of stable amino acid isotopes in cell culture for the purpose of analyzing protein expression through mass spectrometry (MS).
Applications:
• Quantitative and functional proteomics
• Analyses of tissue regeneration
• Analyses of post-translational modifications
• MS (Mass Spectrometry)
• NMR (Nuclear Magnetic Resonance)
General information in pdf