Helios 30S is an ion-exchange resin based on highly cross-linked porous polystyrene-divinylbenzene (PS/DVB) monodisperse microspheres. Chemical compounds are bonded to the surface of the microspheres, giving a highly stable hydrophilic layer. Helios 30S has the characteristics of high flow rate, high resolution, high capacity, high rigidity, good biocompatibility, and physical and chemical stability. Compared with traditional chromatography media, it significantly improves the production efficiency and sample recovery rate of the downstream purification process, and is widely used in the capture, intermediate purification and fine purification of biomolecules such as antibodies, proteins, peptides and nucleic acids.
Products
Helios 30S
No.
114-00025 / 25ml
114-00100 / 100ml
114-00500 / 500ml
114-01000 / 1L
114-05000 / 5L
114-10000 / 10L
114-20000 / 20L
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Chromatography resin parameters
Resin type
Strong cation exchange
Functional group
Sulfomethyl
Matrix
Polystyrene-divinylbenzene
Median particle size
30 μm
Dynamic binding capacity
≥80 mg lysozyme/ml
Pressure flow rate
100–900
Recommended flow rate
300–1000 cm/h
Maximum flow rate
2000 cm/h
Maximum working pressure
40 bar
Chemical resistance
| pH stability* | 2~13 (working range), 1~14 (CIP) |
| Chemical stability | Stable in common aqueous buffers: 1M NaOH+, 1M HCl, 50% acetic acid, 70% ethanol, 8M urea, 6M GuHCl, 30% isopropanol, 0.5% Tween |
| Temperature resistance | Do not freeze! It can tolerate 121°C high-pressure sterilization |
* The physical and chemical properties and functions of the chromatographic resin have no obvious change after being placed in an environment of 40°C and pH 2–12 for 7 days.
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Helios 30S is an ion-exchange resin based on highly cross-linked porous polystyrene-divinylbenzene (PS/DVB) monodisperse microspheres. Chemical compounds are bonded to the surface of the microspheres, giving a highly stable hydrophilic layer. Helios 30S has the characteristics of high flow rate, high resolution, high capacity, high rigidity, good biocompatibility, and physical and chemical stability. Compared with traditional chromatography media, it significantly improves the production efficiency and sample recovery rate of the downstream purification process, and is widely used in the capture, intermediate purification and fine purification of biomolecules such as antibodies, proteins, peptides and nucleic acids.
