Products
Chromatography resins
Please review our currently offered resin lines here: Resin lines
What resins are used for:
- Chromatography resins are widely used for the purification of different types of biomolecules from cells, bacteria and multicomponent solutions: antibodies, enzymes, globulins and other proteins, also lipids, carbohydrates, DNA, RNA and other biomolecules.
- They are used in biotechnology, food technology and agrotechnology, pharmacy and life sciences, in research centers and universities.
What are resins:
- Chromatography resin is tiny beads of agarose or other polymer, coated with functional groups, which interact with specific biomolecule.
- Resins are usually prepacked into chromatographic columns before usage.
- When the cell lysate is flown through the resin inside the chromatographic column the molecule of interest binds to the resin and is separated from all the other molecules found within the cell.
Types of resins
- Size exclusion chromatography (SEC)* – molecules are fractionated by its molecular size;
- Ion exchange chromatography (IEX)* – molecules are fractionated by its charge;
- Hydrophobic interaction chromatography (HIC)* – molecules are fractionated by the level of the surface hydrophobicity;
- Reversed-phase chromatography (RPC)* – used for separating compounds that have even more hydrophobic moieties and do not have a dominant polar character;
- Affinity chromatography (AC)* – molecule interacts specifically with functional group;
- Ceramic hydroxyapatite (CHT)* –separation by calcium and phosphate interactions;
- Mixed-mode chromatography (MMC)* – separation by two or more types of interactions.
The key components of chromatography techniques are the chromatography resins. There is a large diversity of chromatography resin types, including:
- Affinity chromatography
- Hydrophobic interactions chromatography.
- Hydroxyapatite.
- Ion-exchange chromatography.
- Multimodal chromatography.
- Reverse phase chromatography.
- Size-exclusion chromatography.
As the name of each indicates, resins in column chromatography can be used to filter and select molecules depending on a large variety of the characteristics of the target molecules.
As a result, a good understanding of your target molecule is often required to achieve a good level of efficiency and purity in column chromatography.
Affinity chromatography is recommended for purification on the basis of functionality or a unique molecular configuration, with a specific ligand bounded to the material of the chromatography column, binding itself to the target molecule in the mobile phase. This process occurs ideally with an extremely high specificity and high level of affinity, to achieve a high degree of purity in a single step.
Hydrophobic interaction chromatography selects according to the hydrophobicity profile of the molecules present.
Hydroxyapatite is a form of calcium phosphate, which depending on the sintering (liquefaction of a solid) temperature and size of the beads used in the columns, will be able to bind different sizes and types of particles or molecules.
Ion-exchange chromatography selects according to the ionic/electric charge profile of the molecules present.
Multimodal chromatography uses multiple selection methods at the same time, for example, affinity/ligand-based purification in combination with size exclusion or hydrophobicity. This method can reduce the number of steps required for purification by removing several impurities in one go.
Reverse phase chromatography refers to chromatographies where the stationary phase is non-polar, and the mobile phase is polar.
It is the opposite situation of the “normal” liquid chromatography where the stationary phase is polar and the mobile phase is non-polar.
The term “normal” does not indicate the most common method, just the first invented historically, with reverse phase chromatographies now the most commonly done type of chromatography.
Size-exclusion chromatography selects according to the physical size of the molecules present.
The reason for the diversity of resins used in chromatography provided by manufacturers is that every molecule has a unique chemical profile making some techniques work better than others.
Another reason is when purifying a protein or a nucleic acid sequence from biological sources, the medium might vary greatly, making what is being excluded by the resin as important as what is being selected.
These products are designed to provide high binding capacity, selectivity, and resolution, enabling efficient and effective separation of target molecules.
We are among the manufacturers of such resins and can also design custom chromatography resins where the need arises. This is especially relevant for multimodal chromatography, where our design and manufacturing experience will help to anticipate any unexpected and unwanted interaction or incompatibility between materials.
We are also able to advise you on the best type of resins for your purification needs.
Our product can replace USA and Europe top brands manufacturers.
Our experts pay special attention during the manufacturing process to cleaning validation, checking that the manufacturing process leaves no residues, or at least way below the minimum acceptable threshold. Cleaning validation ensures that the resin product is pure, sensitive, and ready to use accurately, and that no contamination occurred.
