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Products

Lepta ProtA LX

Lepta ProtA LX is an affinity chromatography resin for antibody purification, which separates through the specific interaction between antigen and antibody. This chromatography resin is designed to handle high concentrations of monoclonal antibodies. Lepta ProtA LX has the characteristics of high capacity, high flow rate and alkali resistance:

(1) High flow rate and high dynamic binding capacity reduces processing time.

(2) The modified alkali-resistant rProtein A ligand can tolerate 0.5–1.0M NaOH for cleaning-in-place (CIP).

Pleiad CHT (Ceramic Hydroxyapatite) Type II 80μm

No.

150-00025 / 25ml

150-00100 / 100ml

150-00500 / 500ml

150-01000 / 1L

150-05000 / 5L

150-10000 / 10L

Add to order

Chromatography resin parameters

Resin type

Affinity chromatography

Functional group

Alkali-resistant recombinant protein A

Matrix

Highly cross-linked agarose

Median particle size

75 μm

Dynamic binding capacity

>65 mg human IgG/ml *

Maximum flow rate

500 cm/h

Maximum working pressure

5 bar

* Measurement conditions of dynamic binding capacity: packing height, 10 cm; linear flow rate, 100 cm/h; test buffer, 20 mM PB, 0.15M NaCl, pH 7.4 when IgG breakthrough reaches 10% of starting concentration.

Chemical resistance

pH stability*	3–12 (working), 2–14 (CIP)
Chemical stability	Common aqueous solution used in protein A chromatography, 10 mM hydrochloric acid, 0.1M sodium citrate, 6M guanidine hydrochloride, 8M urea, 30% isopropyl alcohol, 20% ethanol

* The physical and chemical properties and functions of the chromatographic resin have no obvious change after being placed in an environment of 40°C and pH 3–12 for 7 days.
** v/v, volume ratio

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Lepta ProtA LX

Chemical resistance

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