Lepta rProtein A is an affinity chromatography resin used for the purification of monoclonal and polyclonal antibodies. Recombinant protein A is produced in E. coli and engineered for directional conjugation to enhance binding capacity, while maintaining specific binding to the IgG Fc fragment. Epoxy-based coupling ensures low ligand shedding.
Products
Lepta rProtein A
No.
652-00025 / 25ml
652-00100 / 100ml
652-00500 / 500ml
652-01000 / 1L
652-05000 / 5L
652-10000 / 10L
652-20000 / 20L
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Chromatography resin parameters
Resin type
Antibody affinity chromatography
Functional group
rProtein A
Matrix
Highly cross-linked agarose
Median particle size
85 μm
Ion load
6 mg rProtein A/ml
Dynamic binding capacity
>35 mg human IgG/ml *
Recommended flow rate
90~500 cm/h
Maximum flow rate
700 cm/h
Maximum working pressure
5 bar
Working temperature
2–40°C
* Measurement conditions of dynamic binding capacity: packing height, 10 cm; retention time, 3 minutes; test buffer, 0.02M NaH2PO4 solution, 0.15M NaCl, pH 7.4 when IgG breakthrough reaches 10% of starting concentration.
Chemical resistance
| pH stability* | 3–12 |
| Chemical stability | Common aqueous buffer solution, 30% isopropanol**, 75% ethanol**, 6M guanidine hydrochloride, 8M urea |
** v/v, volume ratio
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General information in pdf
Lepta rProtein A is an affinity chromatography resin used for the purification of monoclonal and polyclonal antibodies. Recombinant protein A is produced in E. coli and engineered for directional conjugation to enhance binding capacity, while maintaining specific binding to the IgG Fc fragment. Epoxy-based coupling ensures low ligand shedding.
